The effect of various factors on amylase production was examined. Amylase production on submerged fermentation by bacillusspp r. Amylase production by aspergillus niger through submerged fermentation using starchy food byproducts as substrate article pdf available october 2016 with 3,665 reads how we measure reads. Ethanol production and safetyethanol production and safety wei liao, chris saffron. Enzyme production by soud fermentation is known for a long lime. The bacillus strain comprising an alpha amylase gene inserted in the bacillus chromosome under transcriptional control of a phage promoter. Pdf this study evaluated amylase production by bacillus species employing the solid state fermentation ssf method using five. Aspergillus oryzae ls1 produced the highest level of. Results optimization of fermentation parameters for. Optimization of protease and amylase production by rhizopus. The high cost of fermentation media is one of the technical barriers in amylase production from microbial sources. Amylase is a group of enzymes whose catalytic function is to hydrolyze breakdown sugar and starch to give diverse products including dextrins, and progressively smaller polymers composed of glucose units. The results showed that ssf was well suited for developing countries due to costeffective production process kunamneni et al. Production of amylase in liquid culture by a strain of.
The production of amylase by submerged fermentation smf and solid. Historically, fermentation products were mainly food products, but in recent years an increased interest has been observed in the production of bulk chemicals ethanol and other solvents, specialty chemicals pharmaceuticals, industrial enzymes, biofuels and food additives flavor modifiers. Of the carbon sources tested, maltose, sucrose, lactose and soluble starch increased the amylase production by about 180%. In this study, marine microorganisms were isolated to identify unique amylase. Optimization of fermentation parameters for amylase production, the effect on. Amylase production on solid state fermentation by bacillus.
Ethanol production and safetyethanol production and safety. Also, many steps of the metabolic pathways used by yeast are enzymedependent. The production of the enzyme was maximum at 10 h after inoculation. The production of extracellular amylase by bacillus spp was optimized in a submerged fermentation. It is produced mainly from microbial sources and is used in many industries. In submerged culture technique, the yields are more and the chances of infection are less. Amylase production on submerged fermentation by bacillus spp. The a amylase family comprises a group of enzymes with a variety of different specificities that all act on one type of substrate being. Pdf amylase production by solid state fermentation of agro.
All the four fungal isolates were screened for amylase production efficiency in starch agar media comprising the following in gm l1yeast extract 1. Amylase is used in several industrial processes or industries, for example, in the food industry, the saccharification of starchy materials, and in the detergent and textile industry. However, enzymes from fungal and bacterial sources have dominated applications in industrial sectors 29. To study the effect of temperature on amylase production, the submerged fermentation was carried out at different temperatures 25c, 30c, 35c and 40c. The bacillus strain comprising an alphaamylase gene inserted in the bacillus chromosome under transcriptional control of a phage promoter. Fermentative production of enzymesamylase and protease youtube. Amylase is present in the saliva of humans and some other mammals, where it begins the chemical process of digestion. Fermentative production of enzymes amylase and protease. Amylases solid state fermentation are mainly used for traditional food processing and for fungal enzymes production.
Synthesis of amylase by aspergillus niger strain uo01 under solidstate fermentation with sugarcane bagasse was optimized by using response surface methodology and empirical modelling. An efficient process for the rapid production of large amounts of alphaamylase is also disclosed. Steps involved in production of microbial amylase is 1. A novel recombinant bacillus bacterial strain is constructed by genetic engineering which has high productivity of alpha amylase. Pdf amylase and acid protease production by solid state.
The effect of incubation period, ph of the medium and incubation temperature was optimized. Pdf alkaline amylase production by submerged fermentation by. Creation of econtents on fermentation technology project investigator. Amylase production on submerged fermentation by bacillus.
Amylase is an important and indispensable enzyme that plays a pivotal role in the field of biotechnology. Application of microbial amylase in industry starch is hydrolyzed into smaller oligossaccharides by. Industrial sectors with topdown and bottomup approaches are currently focusing on improving microbial amylase production levels by implementing bioengineering technologies. Mar 07, 2014 extracellular amylase production of a thermotolerant fusarium spp.
Industrial production of enzymes is mostly carried out by submerged liquid conditions and to a lesser extent by solidsubstrate fermentation. Amyb was a derivative isoform of amya, sharing practically the same biochemical properties and resulting from proteolytic hydrolysis 11. Amylase production through submerged and solid state fermentation. Amylase is a hydrolase enzyme that catalyses the hydrolysis of internal. Amylase is present in human saliva, where it begins the chemical process of digestion. Pdf the alkali tolerant bacterial isolate bacillus sp. Foods that contain large amounts of starch but little sugar, such as rice and potatoes, may acquire a slightly sweet taste as they are chewed because amylase. Production of glucoamylase by aspergillus oryzae under. The fermentation medium was prepared by varying the ph values 5. Alpha amylase production by aspergillus oryzae employing solidstate fermentation.
The fermentation equipment used for the largescale production of enzymes is the same as that used in the production of other microbial metabolites. The present study was undertaken to isolate, identify and characterize the bacillus subtilis in the culture medium followed by alpha amylase extraction from the fermented carrier, banana peel and bacillus subtilis. Application of microbial amylase in industry amylase has been derived from several fungi, yeasts and bacteria. However, studies reveal that ssf is the most appropriate process in developing countries due to the advantages it offers which make it a cost effective production process. Solid state fermentation which has been reported to be a bit cheaper because of the enzyme extraction procedures 3 is a ray of hope. Fermentation free fulltext amylase production from. Chinese jiuqu fermentation starter provides saccharifying enzymes for baijiu chinese liquor fermentation, which undergoes a simultaneous saccharification and fermentation process. Shivaramakrishnan, 2006 among the various types of amylases produced, commercially thermostable amylases are gaining. Biotechnological processes in microbial amylase production. Synergistic effect of multiple saccharifying enzymes on. Extracellular amylase production of a thermotolerant fusarium spp. Amylaseproduction and process, technology, applications.
A novel recombinant bacillus bacterial strain is constructed by genetic engineering which has high productivity of alphaamylase. Slightly better amylase production resulted with the addition of corn starch, potato starch, rice starch and wheat flour to wheat bran media. Isolation of bacterial fungal strain from various sites and screened by studying their colony morphology and checked for production of alpha amylase by the starch agar plate method described in this lecture at the end. Optimization of amylase production by aspergillus niger in. Pdf amylase production, purification and characterization from. Pdf alpha amylase production by aspergillus oryzae. Submerged fermentation an overview sciencedirect topics. Amylase production by aspergillus niger through submerged fermentation using starchy food byproducts as substrate article pdf available october 2016 with. Alpha amylase is what produces dextrins, which you either already have a lot of, or something went wrong with your yeast seems much more likely to me looking at those numbers. Indhumathi indian institute of crop processing technology, thanjavur 6 005, tamil nadu, india abstract. Further increase in incubation period did not show any significant increase in enzyme production rather it was decreased. Amylase production from aspergillus oryzae ls1 by solid.
Bcc 02150 was found to be the best amylaseproducing strain in starch agar. Next, fermentation conditions were optimized for bacterial growth and enzyme production. Fermentative production of enzymesamylase and protease. Amylase production from aspergillus oryzae ls1 by solidstate. In submerged fermentation the production of amylase was reached maximum of 4 uml at 10 h of incubation period fig. The aamylase family comprises a group of enzymes with a variety of different specificities that all act on one type of substrate. Amylase production on solid state fermentation by bacillus spp. Amylase production, purification and characterization from. The submerged fermentation 3 processes for amylase production have recently 4\ become economically feasible, and a. Pdf amylase production on submerged fermentation by. Us20020187541a1 method for production of alphaamylase in. Ethanol production fermentation ethanol producer yeast yeast are eukaryotic single celled fungi. The production of amylase is essential for conversion of. I should have specified that beta amylase will work if alpha amylase has already exposed the branches from amylopectin that beta amylase could then handle.
However, the key saccharifying enzymes associated with alcoholic fermentation from jiuqu and their effects on ethanol production remain poorly understood. Pdf optimization of amylase production by aspergillus niger. Pdf amylase production on submerged fermentation by bacillus. Penicillium chrysogenum was used as the microbial source for producing amylase by solid state fermentation using various substrates such as, corncob leaf, rye straw, wheat straw and wheat bran 14. Concomitant production of amylase and protease by aspergillus awamori nakazawa mtcc 6652 was enhanced in a single fermentation by media engineering and optimization of other important parameters. Pdf the production of extracellular amylase by bacillus spp was optimized in a submerged fermentation.
Without enzymes, fermentation would not be possible. Production production of amylase was carried out by ssf using the substrates of zero cost namely wheat bran, rice. Early commercial amylase fermentation processes employing a. Production of amylase from banana peels with bacillus. The production of the enzyme was directly related to the growth of the strain. Bcc 02150 per bergeys manual of bacterial determination. Foods that contain much starch but little sugar, such as rice and potato, taste slightly sweet as they are chewed because amylase turns some of their starch into sugar in the mouth. Amylase production by preussia minima, a fungus of. Maximum enzyme activity was obtained at the beginning of the stationary growth phase. Amylase by bacillus subtilis using glucose as carbon source and optimum enzyme activity of culture medium was recorded after 24 h. The fungal source used predominantly for commercial production of. Production of alpha amylases by solid state fermentation using cheap substrates cheap substrates used wheat bran, rice husk, vegetable waste potato, tomato, brinjal and banana peel. Production of enzymes by solidstate fermentation ssf using these moulds turned a costeffective production technique. Optimization of protease and amylase production by.
The latter is a fairly new method while the former is a traditional method of enzyme production from microbes which has been in use for a longer period of time. Ps7 by solidstate fermentation and its synergistic use in the hydrolysis of malt starch for alcohol production, process biochemistry, 402, 525534. The first fungalproduced amylase for industrial application was. Adding alpha amylase to lower fg community beeradvocate. Fermentation processes are also used in agriculture. Aspergillus oryzae s2 alphaamylase production under solid. Amylase is produced in bacteria, fungi, plants and animals.
Alpha amylase production by aspergillus oryzae using solid. After this fermentation time, the activity fell drastically. Factors affecting alfa amylase production on submerged. The highest activity was achieved within 120 h of fermentation for both enzymes 2412 ug and 100 ug, respectively, for protease and amylase. Amylase production on solid state fermentation by bacillus s pp. The process included the culture of aspergillus oryzae on moist rice or wheat. Pdf amylase production by aspergillus niger through. Us20020187541a1 method for production of alphaamylase. Glucoamylase breaks down dextrins into glucose at 60c 2 hr or 30c 48 hr, 4. This invention relates to a novel fermentation process and in particular, to fermentative production of ethanol producing in the presence of nongelled, or granular, starch particles, alphaamylase and a glucoamylase. Agro industrial wastes have been reported to be good substrate for the cost effective production of alpha amylases 4, 5 and are thus attracting researchers for using agro industrial waste as a substrate for alpha amylase production. Solid state fermentation ssf is defined as the cultivation of microorganisms on moist solid supports, either oninert carriers or on insoluble substrates that can, in addition.
Pengthamkeerati p, numsomboon s, satapanajaru t, chairattanamanokorn p. The process parameters tested were particle size of sugarcane bagasse, incubation temperature and ph, moisture level of solid support material and the concentrations. Amylase activity of a starch degrading bacteria isolated. The fermentation medium was prepared by varying ph values5. In this study, marine microorganisms were isolated to identify unique amylase producing microbes. Therefore, ssf is considered as a promising method for commercial enzyme production. The amounts of protease and amylase production were of 1260 ug and 16. In addition, fungal species are highly suitable for solid based fermentation. To meet the higher demands of these industries, low cost production of amylase is required.
1204 827 699 235 1110 1432 562 1518 208 409 200 1534 372 982 842 98 414 1681 189 1242 684 795 1459 1090 1659 1233 1599 1111 534 433 791 480 1499 676 1468 292 754 1304 1065